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human brain large insert cdna library  (TaKaRa)


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    TaKaRa human brain large insert cdna library
    Human Brain Large Insert Cdna Library, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 490 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+brain+large+insert+cdna+library/10__1074_slash_jbc__m109__033969-75-13-19?v=TaKaRa
    Average 94 stars, based on 490 article reviews
    human brain large insert cdna library - by Bioz Stars, 2026-07
    94/100 stars

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    Upregulation of septin 3 isoforms during RA-induced neural differentiation of SH-SY5Y cells. SH-SY5Y cells were treated for 4 days with vehicle (–) or 2.5 μM RA (+). (a) <t>cDNA</t> synthesized from mRNA of treated cells was used as template for PCR. Three sets of primers were used to detect mRNAs for septin <t>3A,</t> <t>3B,</t> and GAPDH as an internal control. The expected sizes of septin 3A (top), 3B (middle), and GAPDH (bottom) mRNAs were 1160, 1308, and 1000 bp, respectively. (b) Lysates from treated cells were separated by 10% SDS-PAGE and then subjected to Western blotting (WB) with antibodies against septin 3A (top), 3B (middle), or β-actin as an internal loading control (bottom).
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    Upregulation of septin 3 isoforms during RA-induced neural differentiation of SH-SY5Y cells. SH-SY5Y cells were treated for 4 days with vehicle (–) or 2.5 μM RA (+). (a) cDNA synthesized from mRNA of treated cells was used as template for PCR. Three sets of primers were used to detect mRNAs for septin 3A, 3B, and GAPDH as an internal control. The expected sizes of septin 3A (top), 3B (middle), and GAPDH (bottom) mRNAs were 1160, 1308, and 1000 bp, respectively. (b) Lysates from treated cells were separated by 10% SDS-PAGE and then subjected to Western blotting (WB) with antibodies against septin 3A (top), 3B (middle), or β-actin as an internal loading control (bottom).

    Journal: Gene Expression

    Article Title: Expression of Septin 3 Isoforms in Human Brain

    doi:

    Figure Lengend Snippet: Upregulation of septin 3 isoforms during RA-induced neural differentiation of SH-SY5Y cells. SH-SY5Y cells were treated for 4 days with vehicle (–) or 2.5 μM RA (+). (a) cDNA synthesized from mRNA of treated cells was used as template for PCR. Three sets of primers were used to detect mRNAs for septin 3A, 3B, and GAPDH as an internal control. The expected sizes of septin 3A (top), 3B (middle), and GAPDH (bottom) mRNAs were 1160, 1308, and 1000 bp, respectively. (b) Lysates from treated cells were separated by 10% SDS-PAGE and then subjected to Western blotting (WB) with antibodies against septin 3A (top), 3B (middle), or β-actin as an internal loading control (bottom).

    Article Snippet: The human cDNA fragments of septin 3A, 3B, and Nedd5 were produced by polymerase chain reaction (PCR) from the Human Fetal Brain Large-Insert cDNA library (BD Biosciences Clontech, Palo Alto, CA).

    Techniques: Synthesized, SDS Page, Western Blot